产品货号:
Z11773
中文名称:
BCKDHA抗体
英文名称:
Anti-BCKDHA Antibody
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称 | Anti-BCKDHA Antibody |
指标别名 | BCKDHA;FLJ45695;MSU;MSUD1;OVD1A |
克隆性 | Polyclonal |
检验物种 | human,mouse,rat |
应用范围 | WB,IHC,ICC/IF,FCM,ELISA |
基因名称 | BCKDHA |
抗体来源 | Rabbit |
抗体类型 | IgG |
免疫原 | E.coli-derived human BCKDHA recombinant protein (Position:A30-K445). |
实际分子量 | 50KD |
成分 | 500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500μg/mL |
产品形态 | 溶液 |
保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
背景资料 | 2-oxoisovalerate dehydrogenase subunit alpha,mitochondrial is an enzyme that in humans is encoded by the BCKDHA gene.The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine,isoleucine,and valine.The BCKD complex consists of three catalytic components:a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1),a dihydrolipoyl transacylase (E2),and a dihydrolipoamide dehydrogenase (E3).This gene encodes the alpha subunit of the decarboxylase (E1) component.Mutations in this gene result in maple syrup urine disease,type IA.Multiple transcript variants encoding different isoforms have been found for this gene. |
Uniprot ID | P12694 |
推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot. |
基因名全称 | branched chain keto acid dehydrogenase E1 subunit alpha |
蛋白名全称 | 2-oxoisovalerate dehydrogenase subunit alpha,mitochondrial |
推荐稀释比 | Western blot (WB) 1:500~2000 Immunohistochemistry (IHC) 1:50~400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50~400 Flow cytometry (FCM) 1~3μg/1×106 cells Enzyme linked immunosorbent assay (ELISA) 1:100~1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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